However, the majority of these effects were more pronounced or earlier detected in Wistar rats. It is unknown to what extent impaired glucose tolerance directly causes nerve injury or is simply a covariant with other factors such as obesity It is possible that in the vasculature of resistance vessels, pathways linked to insulin action are upregulated in obesity.
Weight put on during the high-fat diet also tends to persist. To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. Interestingly, NEP activity is also increased in skin of patients with diabetic ulcers In the past decades, obesity and associated metabolic complications have reached epidemic proportions.
The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR. For instance, casein causes more weight gaining compared to soy.
The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats. While models are an important method of investigating the diet induced obesity sparague of obesity and drug testing, it is important to understand the limits of the model's overall ability to resemble the human obesogenic pathophysiology.
Researchers have created the study models of high carbohydrate and high proteins. If our in vitro vascular response data to insulin can be translated to in vivo conditions, it would suggest that at levels of diet induced obesity sparague seen in control rats, vascular relaxation due to insulin would be negligible.
Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet. For the study of these pathologies, a number of animal models have been developed. The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats.
The researchers found that the mice injected with propolis had less adipose tissueglucoseand cholesterol than the mice who were not administered propolis. Data in Figure 8 demonstrate that expression of NEP was increased about 2.
For instance, the age at which mice begin the high-fat diet greatly impacts the metabolic effects. The body weight gain is quantified using the difference in the raw mass of the animal or in the Lee index an index similar to the BMI in humans.
The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR. SNCV was significantly decreased in rats fed a high fat diet for 24 and 32 weeks Figure 2 C hatched bars. One such characteristic is insulin resistancewhich comes with diet-induced obesity in both rats and humans.
The results of the study illustrate that the high fat diet rats had a higher adiposity index than the low fat diet rats. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats.
A qualitative assessment of sciatic nerves by light microscopy did not identify any overt pathological damage to axons or any changes in myelin structure such as splitting, ballooning or thinning.
However, this association should be interpreted with caution as thermal hypoalgesia also occurs in mice fed a high fat diet in the absence of intraepidermal nerve fibre loss 41and thermal hypoalgesia precedes intraepidermal nerve fibre loss in diabetic mice It is possible that in vivo the reduced vascular relaxation in response to acetylcholine and CGRP is compensated by the increased response to insulin, resulting in the maintenance of endoneurial blood flow.
Figure 4 Open in figure viewer PowerPoint Effect of a high fat diet on neutral endopeptidase activity NEP in the skin of the hindpaw Rats were fed a standard or high fat diet for 32 weeks.
For example, mice were put on a high-fat diet, but given either tap water, green tea, or Goishi tea to drink. Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet.
The blue arrow pointing at the vessel in the upper right portion of the image on the left side is enlarged and shown on the right side. For the study of these pathologies, a number of animal models have been developed.RESEARCH PAPER High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat Claudia Marques a, b, Manuela Meireles a,Sonia Norberto, Joana Leite, Joana Freitas,c, Diogo Pestana.
Therefore, the diet-induced obese Sprague-Dawley rat may serve as a suitable model for evaluating toxicity findings encountered with anti-obesity compounds. Keywords Sprague-Dawley rat, diet-induced obesity, toxicology parameters, bone toxicity, reproductive toxicity, Author: Jennifer M.
Rojas, Florian Bolze, Inger Thorup, Jette Nowak, Charlotte M. Dalsgaard, Mikala Skydsgaa. Cláudia Marques, Manuela Meireles, Sónia Norberto, Joana Leite, Joana Freitas, Diogo Pestana, Ana Faria and Conceição Calhau, High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat, Adipocyte, 5, 1, (11), ().Cited by: In outbred Sprague-Dawley rats, about one-half develop diet-induced obesity (DIO) on a diet relatively high in fat and energy (HE diet).
The rest are diet resistant (DR), gaining weight and fat at the same rate as chow-fed elbfrollein.com by: Environment-Induced Obesity. In addition to genetic mutation-induced obese animal models, diet-induced obesity (DIO) is also widely accepted to study the mechanisms that contribute to obesity-induced cardiac injury and dysfunction (Sciarretta et al., ; Xu et al., ).
The consumption of a high energy (HE) diet causes a range of weight gain responses in the human population with some individuals resistant to diet induced obesity (DIO).Author: Z Archer, D Rayner, J Mercer.